Journal: Frontiers in Immunology
Article Title: Arginine-mediated inhibition of macrophage apoptosis by Escherichia coli nissle 1917 in Salmonella typhimurium-induced intestinal inflammation
doi: 10.3389/fimmu.2025.1684234
Figure Lengend Snippet: Arginine inhibits S. Typhimurium infection by interacting with RPS3 (-B) Enrichment analysis of RAW264.7 cells cell lysate and arginine binding protein , pathway (A) and heatmap of protein abundance associated with Salmonella infection ( B, C ) Knockdown efficiency of RPS3 in RAW264.7 cell line was verified by Western blotting ( n = 5 independent experiments). (D) DCFH-DA fluorescent probe assay for detection of cellular ROS Levels ( n = 5 independent experiments). (E) Representative confocal microscopy image illustrating the mitochondrial abundance within macrophages ( n = 5 independent experiments). (F) Venn diagram of RPS3 binding, arginine metabolism, and protein overlap associated with Salmonella infection. (G) Interactions between RPS3 interaction with SRSF3 was analyzed using the GeneMANIA database. (H) RPS3 and SRSF3 by co-immunoprecipitation assays using anti-SRSF3 antibodies. The data are reported as the mean ± SD. **p < 0.01.
Article Snippet: Cellular collectives were fixed with 2.5% glutaraldehyde for 24 h, embedded in 2% agarose, and processed by the Electron Microscopy Research Service of China Pharmaceutical University.
Techniques: Infection, Binding Assay, Quantitative Proteomics, Knockdown, Western Blot, Confocal Microscopy, Immunoprecipitation